Chemical Characterization and Biological Activities of Citrus reticulata and Citrus aurantium Essential Oils

  • Nouara Ait-Mimoune Department of Biology, Bouira University, Bouira, Algeria
  • Karima Bouti Department of sciences, Ecole Normale Supérieure de Kouba, Algiers, Algeria
  • Bouchra Benabdi Department of Biology, Bouira University, Bouira, Algeria
  • Ahlam Otmani Department of Biology, Bouira University, Bouira, Algeria
Keywords: Antifungal, Antibacterial, Antioxidant, Citrus, GC/MS


This study was conducted to evaluate the chemical composition, antimicrobial and antioxidant activities of Citrus reticulata and Citrus aurantium essential oils (EOs). The chemical composition was determined by gas chromatography-mass spectrometry (GC/MS). Monoterpene hydrocarbons were found to be the main components. Antibacterial activity was evaluated against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa. The Gram-positive bacteria Staphylococcus aureus was more sensitive than the Gram-negative bacteria. Antifungal properties were determined against Aspergillus and Penicillium species. The best results were obtained by Citrus reticulata and proved to be the most effective inhibitor of all tested fungi. In addition, the screening of the antioxidant activities showed that Citrus aurantium was more effective in scavenging DPPH radicals. A reduction in the IC50 values was also noticed when the EOs were used in combination. According to these findings, the tested EOs could be used as a natural source of new compounds for the treatment of fungal and bacterial infections.


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How to Cite
Ait-Mimoune, N., Bouti, K., Benabdi, B., & Otmani, A. (2022). Chemical Characterization and Biological Activities of Citrus reticulata and Citrus aurantium Essential Oils. Journal of Environmental Treatment Techniques, 10(2), 182-186.
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