Bioremediation of Synthetic Pyrethroid by Hydrolases of Bacillus aryabhattai and Bacillus circulans Derived from Indigenous Soil
Synthetic pyrethroids are widely used for the improvement of crop production but are also regarded as potentially harmful pollutants. The present study aimed to evaluate the efficiency of different indigenous soil bacteria to degrade Lambda Cyhalothrin (LC), a known synthetic pyrethroid. The sampling area was selected as Rawal Lake and soil samples were collected from alongside upper streams that flow into Lake. LC degrading bacterial strains were isolated and identified as Bacillus aryabhattai and Bacillus circulans. COD was used as a parameter for measuring % the removal of LC at different intervals. Bacillus aryabhattai showed % removal of 55% whereas, Bacillus circulans showed a % removal of 83% in Minimal Salt Media after 72 hours. This study revealed that Bacillus circulans may tolerate LC more effectively at higher concentrations and therefore may be used as potential hydrolyzing enzymes that may disrupt chemical bonds of pyrethroid and result in the reduction of toxicity. This work exhibited a promising approach for the bioremediation of LC and may hence be used as environmental bioremediations of other pyrethroids as well.
Ayaz, M., Sharif, M., Ayesha, B., Tahir, N., Ali, S., Ahmed, S., & Ahmed, N. (2016). Determination of water quality of Rawal Dam, Islamabad. Academia Journal of Agricultural Research, 4(3), 113-117.
Abdullah, R.R., Ghani, S.B.A., & Sukar, N.A. (2016) Degradation of profenofos and λ-cyhalothrin using endogenous bacterial isolates and detection of the responsible genes. J Bioremediat Biodegrad 7, 360.
APHA (American Public Health Association Manual) (2017). Standards methods for the examination of water and wastewater.
Bodor A, Bounedjoum N, Vincze GE, Erdeiné Kis Á, Laczi K, Bende G, Szilágyi Á, Kovács T, Perei K, Rákhely G. Challenges of unculturable bacteria: environmental perspectives. Reviews in Environmental Science and Bio/Technology. 2020 Mar;19(1):1-22.
Bhatt, P., Huang, Y., Zhan, H., Chen, S., 2019. Insight into microbial applications for the biodegradation of pyrethroid insecticides. Front. Microbiol. 10, 1–19.
Bhatt, P., Huang, Y., Rene, E.R., Kumar, A.J., Chen, S., 2020a. Mechanism of allethrin biodegradation by a newly isolated Sphingomonas trueperi strain CW3 from wastewater sludge. Bioresour. Technol. 305, 123074–123083
Bhatt, P., Bhatt, K., Huang, Y., Lin, Z., Chen, S., 2020b. Esterase is a powerful tool for the biodegradation of pyrethroid insecticides. Chemosphere 244, 125507–561125521.
Bhatt, P., Zhou, X., Huang, Y., Zhang, W., Chen, S., 2021a. Characterization of the role of esterases in the biodegradation of organophosphate, carbamate, and pyrethroid pesticides. J. Hazard Mater. 411, 125026–125046.
Birolli, W. G., Vacondio, B., Alvarenga, N., Seleghim, M. H., & Porto, A. L. (2018). Enantioselective biodegradation of the pyrethroid (±)-lambda-cyhalothrin by marine-derived fungi. Chemosphere, 197, 651-660.
Chen, S., Deng, Y., Chang, C., Lee, J., Cheng, Y., Cui, Z., & Zhang, L. H. (2015). Pathway and kinetics of cyhalothrin biodegradation by Bacillus thuringiensis strain ZS-19. Scientific Reports, 5, 8784.
Collis, W.M.D., Leahey, J.P., 1984. Syngenta Report No RJ0338B: Hydrolysis in Water at pH 5, 7 and 9. Syngenta, Berkshire, United Kingdom. Collins, C.H & Lyne, P.M. (1985). Microbiological Methods, 5th Edition. Butterwood and Co (Publishers) Ltd.
Cycon, M., Piotrowska-Seget, Z., 2016. Pyrethroid-degrading microorganisms and their potential for the bioremediation of contaminated soils: a review. Front.Microbiol. 7, 1463.
Cycoń M, Mrozik A, Piotrowska-Seget Z. Bioaugmentation as a strategy for the remediation of pesticide-polluted soil: A review. Chemosphere. 2017 Apr 1;172:52-71.
Dehghani MH, Karri RR, Anastopoulos I. Pesticides Remediation Technologies from Water and Wastewater (2022).
Ding J, Liu Y, Gao Y, Zhang C, Wang Y, Xu B, Yang Y, Wu Q, Huang Z. Biodegradation of λ-cyhalothrin through cell surface display of bacterial carboxylesterase. Chemosphere. 2022 Feb 1;289:133130.
Erguven, G. O., & Yildirim, N. (2016). Efficiency of some soil bacteria for chemical oxygen demand reduction of synthetic chlorsulfuron solutions under agitated culture conditions. Cellular and Molecular Biology, 62(6), 92-96.
Galadima M, Singh S, Pawar A, Khasnabis S, Dhanjal DS, Anil AG, Rai P, Ramamurthy PC, Singh J. Toxicity, microbial degradation and analytical detection of pyrethroids: a review. Environmental Advances. 2021 Oct 1;5:100105.
Gonçalves CR, da Silva Delabona P. Bioremediation of pesticides in Brazil: a brief overview. Environmental Advances. 2022 Apr 1:100220.
Ghumro, W. A., Qazi, M. A., & Kanhar, N. A. (2017). Pesticide Lambda-cyhalothrin degradation using Mesorhizobium sp. (S1b) and Bartonella sp. (S2b) strains isolated from cotton crop. Pak. J. Anal. Environ. Chem, Vol. 18(2), 112 – 119.
Gong, T., Xu, X., Dang, Y., Kong, A., Wu, Y., Liang, P., Wang, S., Yu, H., Xu, P., Yang, C., 2018. An engineered Pseudomonas putida can simultaneously degrade organophosphates, pyrethroids and carbamates. Sci. Total Environ. 628–629, 1258–1265.
Guo, P., Wang, B., Hang, B.J., Li, L., Ali, S.W., He, J., Li, S., 2009. Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase. Int. Biodeterior. Biodegrad. 63, 1107–1112.
He, L.M., Troiano, J., Wang, A., Goh, K., 2008. Environmental chemistry, ecotoxicity, and fate of lambda-cyhalothrin. Rev. Environ. Contam. Toxicol. 195, 71e91.
Housset, P., Dickman, R., 2009. A promise fulfilled e pyrethroid development and the benefits for agriculture and human health. Bayer Crop Sci. J. 62, 135e144.
Khalid, S., Hashmi, I., & Khan, S. J. (2016). Bacterial assisted degradation of chlorpyrifos: The key role of environmental conditions, trace metals and organic solvents. Journal of environmental management, 168, 1-9.
Khalid. S, & Hashmi. I. (2016). Biotreatment of chlorpyrifos in a bench scale bioreactor using Psychrobacter alimentarius T14. Environmental Technology, 37(3), 316-325.
Khan, Imran. M, Muhammad. S, Sardar. A, Arif. N, Nabeel. N, Muhammad. A, Safdar. B, Imran. A, & Rashad. Q. (2018). Use, contamination and exposure of pesticides in Pakistan: A review.
Pakistan Journal of Agricultural Sciences. 55.
Kumari, B.L., Hanumasri, M., & Sudhakar, P. (2011). Isolation of cellulase producing fungi from soil, optimization and molecular characterization of the isolate for maximizing the enzyme yield.
World Journal of Science and Technology, 1(5).
Liu J, Huang W, Han H, She C, Zhong G. Characterization of cell-free extracts from fenpropathrin-degrading strain Bacillus cereus ZH-3 and its potential for bioremediation of pyrethroid-contaminated soils. Science of the Total Environment. 2015 Aug 1;523:50-8.
Rayu, S., Nielsen, U. N., Nazaries, L., & Singh, B. K. (2017). Isolation and molecular characterization of novel chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol-degrading bacteria from sugarcane farm soils. Frontiers in Microbiology, 8.
Sarker A, Nandi R, Kim JE, Islam T. Remediation of chemical pesticides from contaminated sites through potential microorganisms and their functional enzymes: Prospects and challenges. Environmental Technology & Innovation. 2021 Aug 1;23:101777.
Saillenfait, A.M., Ndiaye, D., Sabate, J.P., 2015. Pyrethroids: exposure and health effects - an update. Int. J. Hyg Environ. Health 218, 281e292.
Sharma, I. (2020). Bioremediation techniques for polluted environment: concept, advantages, limitations, and prospects. In Trace Metals in the Environment-New Approaches and Recent Advances. IntechOpen.
Tran KM, Lee HM, Thai TD, Shen J, Eyun SI, Na D. Synthetically engineered microbial scavengers for enhanced bioremediation. Journal of Hazardous Materials. 2021 Oct 5;419:126516.
Thatheyus, A. J., & Selvam, A. D. G. (2013). Synthetic pyrethroids: toxicity and biodegradation. Appl Ecol Environ Sci, 1(3), 33-6.
Zhai, Y., Li, K., Song, J., Shi, Y., Yan, Y., 2012. Molecular cloning, purification and biochemical characterization of a novel pyrethroid-hydrolyzing carboxylesterase gene from Ochrobactrum anthropi YZ-1. J. Hazard Mater. 221–222, 206–212.
Zhan, H., Huang, Y., Lin, Z., Bhatt, P., Chen, S., 2020. New insights into the microbial degradation and catalytic mechanism of synthetic pyrethroids. Environ. Res. 182, 109138–109149.
Zhang, R., Zhou, Z., & Feng, J. (2016). Isolation, identification, and characterization of Lambdacyhalothrin pesticide degrading bacterium ZC-5. Key Engineering Materials. Vol. 723, pp 628632.
Zhang, R. H., Zhou, Z. H., & Feng, J. C. (2017). Isolation, Identification, and Characterization of Lambda-Cyhalothrin Pesticide Degrading Bacterium ZC-5. In Key Engineering Materials (Vol. 723, pp. 628-632). Trans Tech Publications Ltd.
Zhao T, Hu K, Li J, Zhu Y, Liu A, Yao K, Liu S. Current insights into the microbial degradation for pyrethroids: strain safety, biochemical pathway, and genetic engineering. Chemosphere. 2021 Sep 1;279:130542.
Zheng, L. L., Mou, H. J., & Li, J. (2012). Determination and microbial degradation of lambda-cyhalothrin. In Advanced Materials Research (Vol. 343, pp. 430-437). Trans Tech Publications Ltd.
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