Journal of Infertility and Reproductive Biology 2022-05-20T00:58:43-05:00 Dr. Sanaz Alaee Open Journal Systems <p><strong><span lang="EN-GB">Journal of Infertility and Reproductive Biology (JIRB)</span></strong></p> <p><strong><span lang="EN-GB">ISSN:</span></strong><span lang="EN-GB">&nbsp;2310-7588</span></p> <p style="text-align: justify;">Journal of Infertility and Reproductive Biology (JIRB) is a quarterly, peer-reviewed, open access, and free of charge journal that publishes original research articles as well as review articles in all areas of infertility and reproductive sciences. This journal was financed by&nbsp;<span class="SpellE">D</span><span class="SpellE">orma</span>&nbsp;Trading&nbsp;<span class="SpellE">Est</span>, publisher<a href="">,</a>&nbsp;Dubai, United Arab Emirates, and Jami Institute of Technology, Isfahan, Iran.&nbsp;JIRB is an Open Access Journal Database&nbsp;<span class="il">for</span>&nbsp;the high visibility and promotion of your articles. JIRB is steered by a distinguished&nbsp;<a href="">Board of Editors</a>&nbsp;and is supported by an international reviewer team consisting of prominent individuals representing many well-known universities, colleges, and corporations in Germany, Spain, Korea, India, Bangladesh, and many more.<strong>&nbsp;</strong>To maintain a high-quality journal, manuscripts that appear in the JIRB Articles section have been subjected to a rigorous review process. This includes blind reviews by three or more members of the international editorial review team, followed by a detailed review by the JIRB editors. The JIRB has been financially and scientifically supported by <a href="">the Jami Institute of Technology</a>. The Journal welcomes original research contributions (previously unpublished) from all established areas of infertility and reproductive sciences.&nbsp;</p> Comparison between Sperm Preparation before Cryopreservation and Post-Thawing Sperm Preparation using Density Gradient Centrifugation in Normozoospermic Semen Samples 2022-05-20T00:58:43-05:00 Moungala Lionel Wildy <p>Sperm cryopreservation has been considered as an&nbsp;efficient procedure for management of male fertility. Different freezing protocols have been developed to maximize the post freeze quality. The current study investigates the differences between sperm preparation before and after freeze. 120 Semen analysis reports from 18 sperm donors were analysed. 60 semen analysis reports were from samples that were frozen then washed (Group 1: Post-wash) while the other 60 semen analysis reports were obtained from samples that were washed then frozen (Group 2: Pre-wash). Sperm concentration and progressive motility were evaluated for each group and compared. Sperm concentration was higher in the pre-wash group compared to post-wash (33.05 ±12.9 vs 26.13 ±13.36, respectively). Progressive motility was higher in the post-wash group (82.3 ±14.9 vs 51.6 ±8.2). There was a significant decrease in sperm concentration between fresh samples and both (post- and pre-wash) results. Significant decreases in progressive motility were found in both methods. Sperm freeze using fresh semen samples and washed samples resulted in significant decrease in post freeze progressive motility. However, fresh samples had a higher post freeze recovery rate compared to washed samples (72.15% vs 56.32%). Semen preparation by density gradient centrifugation before freezing resulted in better sperm concentration, while the preparation of spermatozoa after freezing had a higher progressive motility. Therefore, sperm freezing methods should be adapted according to semen samples quality.</p> 2022-05-20T00:58:43-05:00 ##submission.copyrightStatement##