Recent advances in nano-biotechnology for breast cancer therapy

  • Fatemeh Madadi Atmian Sofla Department of Chemical Engineering, University of Mohaghegh Ardabili, Ardabil, Iran
  • Aziz Babapoor Department of Chemical Engineering, University of Mohaghegh Ardabili, Ardabil, Iran
Keywords: Breast cancer, Nano-biotechnology, Nanoparticle, and Nano-drug delivery system


 One of the most common cancers in the world is breast cancer. The incidence of breast cancer has increased by more than 30% in the past 25 years, despite a significant decrease in fatality. On the other hand, due to the many advances in technology, even the best treatments for all types of cancer, including breast cancer, accessible today are not 100% efficacious. Therefore, many scientists are exploring new strategies based on nanotechnology to access more suitable options for breast cancer treatment and diagnosis. In recent years, to develop nanomaterials with unique properties many efforts have been made. several of these attributes are used to expand tools to diagnose and treat cancers, including breast cancer. Nano-biotechnology and nano-drug delivery systems to cancer cells are a new method with the capability to enhance the immune system to identify and annihilate cancer cells with high selectivity. As a promising treatment, nano-medicines can not only eliminate primary tumors but are also effective in preventing metastasis and recurrence. Although much advance has been made, significant challenges yet need to be addressed. The purpose of this article is to review recent studies on the clinical therapy of breast cancer and provide new concepts about nano-drug transfer systems for anti-breast cancer drugs. Also, we review the main areas of nano-biotechnology research.



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1. Prabhakar, U., et al., Challenges and Key Considerations of the Enhanced Permeability and Retention Effect for Nanomedicine Drug Delivery in OncologyEPR Effect and Nanomedicine Drug Delivery in Oncology. Cancer research, 2013. 73(8): p. 2412-2417.
2. Mousavi, S.M., et al., Bioactive graphene quantum dots based polymer composite for biomedical applications. Polymers, 2022. 14(3): p. 617.
3. Kalashgarani, M.Y. and A. Babapoor, Application of nano-antibiotics in the diagnosis and treatment of infectious diseases. Advances in Applied NanoBio-Technologies, 2022. 3(1): p. 22-35.
4. Rocha, M., N. Chaves, and S. Báo, Nanobiotechnology for breast cancer treatment. Breast Cancer-From Biology to Medicine, 2017.
5. Mousavi, S.M., et al., Plasma-Enabled Smart Nanoexosome Platform as Emerging Immunopathogenesis for Clinical Viral Infection. Pharmaceutics, 2022. 14(5): p. 1054.
6. Alipour, A. and M.Y. Kalashgarani, Nano Protein and Peptides for Drug Delivery and Anticancer Agents. Advances in Applied NanoBio-Technologies, 2022. 3(1): p. 60-64.
7. Al-Ajmi, K., et al., Review of non-clinical risk models to aid prevention of breast cancer. Cancer Causes & Control, 2018. 29(10): p. 967-986.
8. Sánchez-Jiménez, F., et al., Obesity and breast cancer: role of leptin. Frontiers in oncology, 2019. 9: p. 596.
9. Mousavi, S.M., et al., Recent advances in plasma-engineered polymers for biomarker-based viral detection and highly multiplexed analysis. Biosensors, 2022. 12(5): p. 286.
10. Kazemi, K., Y. Ghahramani, and M.Y. Kalashgrani, Nano biofilms: An emerging biotechnology applications. Advances in Applied NanoBio-Technologies, 2022. 3(2): p. 8-15.
11. Bahreyni, A., Y. Mohamud, and H. Luo, Emerging nanomedicines for effective breast cancer immunotherapy. Journal of nanobiotechnology, 2020. 18(1): p. 1-14.
12. Kalashgrani, M.Y. and N. Javanmardi, Multifunctional Gold nanoparticle: As novel agents for cancer treatment. Advances in Applied NanoBio-Technologies, 2022: p. 1-6.
13. Mousavi, S.M., et al., The Pivotal Role of Quantum Dots-Based Biomarkers Integrated with Ultra-Sensitive Probes for Multiplex Detection of Human Viral Infections. Pharmaceuticals, 2022. 15(7): p. 880.
14. Fang, X., J. Cao, and A. Shen, Advances in anti-breast cancer drugs and the application of nano-drug delivery systems in breast cancer therapy. Journal of Drug Delivery Science and Technology, 2020. 57: p. 101662.
15. Kalashgrani, M.Y., F.F. Nejad, and V. Rahmanian, Carbon Quantum Dots Platforms: As nano therapeutic for Biomedical Applications. Advances in Applied NanoBio-Technologies, 2022. 3(2): p. 38-42.
16. Mousavi, S.M., et al., Highly sensitive flexible SERS-based sensing platform for detection of COVID-19. Biosensors, 2022. 12(7): p. 466.
17. Wang, P., Y. Du, and J. Wang, Indentification of breast cancer subtypes sensitive to HCQ-induced autophagy inhibition. Pathology-Research and Practice, 2019. 215(10): p. 152609.
18. Kalashgrani, M.Y., et al., Recent Advances in Multifunctional magnetic nano platform for Biomedical Applications: A mini review. Advances in Applied NanoBio-Technologies, 2022. 3(2): p. 31-37.
19. Mousavi, S.M., et al., Recent Advances in Inflammatory Diagnosis with Graphene Quantum Dots Enhanced SERS Detection. Biosensors, 2022. 12(7): p. 461.
20. Tao, Z., et al., Breast cancer: epidemiology and etiology. Cell biochemistry and biophysics, 2015. 72(2): p. 333-338.
21. Haghighat, S., et al., Standardized breast cancer mortality rate compared to the general female population of Iran. Asian Pacific Journal of Cancer Prevention, 2012. 13(11): p. 5525-5528.
22. Mousavi, S.M., et al., Biomedical Applications of an Ultra-Sensitive Surface Plasmon Resonance Biosensor Based on Smart MXene Quantum Dots (SMQDs). Biosensors, 2022. 12(9): p. 743.
23. Mousavi, S.M., et al., Bioresource-Functionalized Quantum Dots for Energy Generation and Storage: Recent Advances and Feature Perspective. Nanomaterials, 2022. 12(21): p. 3905.
24. Mansfield, C.M., A review of the etiology of breast cancer. Journal of the National Medical Association, 1993. 85(3): p. 217.
25. Mansourian, R., et al., CeO2/TiO2/SiO2 nanocatalyst for the photocatalytic and sonophotocatalytic degradation of chlorpyrifos. The Canadian Journal of Chemical Engineering, 2022. 100(3): p. 451-464.
26. Mousavi, S.M., et al., Antiproliferative and apoptotic effects of graphene oxide@ AlFu MOF based saponin natural product on OSCC line. Pharmaceuticals, 2022. 15(9): p. 1137.
27. Wielsøe, M., S. Gudmundsdottir, and E. Bonefeld-Jørgensen, Reproductive history and dietary habits and breast cancer risk in Greenlandic Inuit: a case control study. Public Health, 2016. 137: p. 50-58.
28. Hanf, V. and D. Hanf, Reproduction and breast cancer risk. Breast care, 2014. 9(6): p. 398-405.
29. Namiranian, N., et al., Risk factors of breast cancer in the Eastern Mediterranean Region: a systematic review and meta-analysis. Asian Pacific Journal of Cancer Prevention, 2014. 15(21): p. 9535-9541.
30. Mousavi, S.M., et al., Hybrid of sodium polytungstate polyoxometalate supported by the green substrate for photocatalytic degradation of auramine-O dye. Environmental Science and Pollution Research, 2022. 29(37): p. 56055-56067.
31. Wang, X., et al., The association between body size and breast cancer in Han women in Northern and Eastern China. The oncologist, 2016. 21(11): p. 1362-1368.
32. Shield, K.D., I. Soerjomataram, and J. Rehm, Alcohol use and breast cancer: a critical review. Alcoholism: Clinical and Experimental Research, 2016. 40(6): p. 1166-1181.
33. Arshadi, M., et al., Green recovery of Cu-Ni-Fe from a mixture of spent PCBs using adapted A. ferrooxidans in a bubble column bioreactor. Separation and Purification Technology, 2021. 272: p. 118701.
34. Golzar-Ahmadi, M. and S.M. Mousavi, Extraction of valuable metals from discarded AMOLED displays in smartphones using Bacillus foraminis as an alkali-tolerant strain. Waste Management, 2021. 131: p. 226-236.
35. White, B.E., et al., Nanotechnology approaches to addressing HER2-positive breast cancer. Cancer Nanotechnology, 2020. 11(1): p. 1-26.
36. Pourhossein, F., et al., Novel green hybrid acidic-cyanide bioleaching applied for high recovery of precious and critical metals from spent light emitting diode lamps. Journal of Cleaner Production, 2021. 298: p. 126714.
37. Abdollahifar, A., et al., Fabrication of graphene oxide‐lead oxide epoxy based composite with enhanced chemical resistance, hydrophobicity and thermo‐mechanical properties. Advances in Polymer Technology, 2018. 37(8): p. 3792-3803.
38. Senkus, E., et al., Primary breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Annals of oncology, 2015. 26: p. v8-v30.
39. Hejazian, S.H., et al., Protection against brain tissues oxidative damage as a possible mechanism for improving effects of low doses of estradiol on scopolamine-induced learning and memory impairments in ovariectomized rats. Advanced biomedical research, 2016. 5.
40. Hashemi, S.A., et al., Picomolar-level detection of mercury within non-biological/biological aqueous media using ultra-sensitive polyaniline-Fe 3 O 4-silver diethyldithiocarbamate nanostructure. Analytical and Bioanalytical Chemistry, 2020. 412: p. 5353-5365.
41. Buchholz, T.A., Radiation therapy for early-stage breast cancer after breast-conserving surgery. New England Journal of Medicine, 2009. 360(1): p. 63-70.
42. Florescu, A., et al., Immune therapy for breast cancer in 2010—hype or hope? Current oncology, 2011. 18(1): p. 623.
43. Abbasnezhad, A., et al., Comparison the effect of hydroalcholic extract of nigella sativa L. seed and metformin on blood biochemical parameters in streptozotocin-induced Diabetic rats. Internal Medicine Today, 2015. 20(4): p. 243-248.
44. Sephton, S.E., et al., Depression, cortisol, and suppressed cell-mediated immunity in metastatic breast cancer. Brain, behavior, and immunity, 2009. 23(8): p. 1148-1155.
45. Esmaeili, H., et al., Activated carbon@ MgO@ Fe 3 O 4 as an efficient adsorbent for As (III) removal. Carbon Letters, 2021. 31: p. 851-862.
46. Hosseini, H. and S.M. Mousavi, Density functional theory simulation for Cr (VI) removal from wastewater using bacterial cellulose/polyaniline. International Journal of Biological Macromolecules, 2020. 165: p. 883-901.
47. Jain, V., et al., A review of nanotechnology-based approaches for breast cancer and triple-negative breast cancer. Journal of Controlled Release, 2020. 326: p. 628-647.
48. Hashemi, M., et al., Evaluation of Ca-independent α-amylase production by Bacillus sp. KR-8104 in submerged and solid state fermentation systems. 2011.
49. Arjmand, O., et al., Polyvinyl alcohol with superior flooding properties to enhance oil recovery process. Research Journal of Applied Sciences, Engineering and Technology, 2012. 4(17): p. 3062-3064.
50. Mousavi, S., et al., Modifying the properties of polypropylene-wood composite by natural polymers and eggshell Nano-particles. Polymers from Renewable Resources, 2015. 6(4): p. 157-173.
51. Hosseini, H., et al., Display of hidden properties of flexible aerogel based on bacterial cellulose/polyaniline nanocomposites with helping of multiscale modeling. European polymer journal, 2021. 146: p. 110251.
52. Zackrisson, S. and F. Cardoso, Guidelines, E (2015). clinical practice guidelines Primary breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up† clinical practice guidelines. Annals of Oncology. 26(5): p. 8-30.
53. Mousavi, S.M., et al., Separation of Ni (II) from industrial wastewater by kombucha scoby as a colony consisted from bacteria and yeast: kinetic and equilibrium studies. Acta Chimica Slovenica, 2019. 66(4): p. 865-873.
54. Khademolhosseini, R., et al., Investigation of synergistic effects between silica nanoparticles, biosurfactant and salinity in simultaneous flooding for enhanced oil recovery. RSC advances, 2019. 9(35): p. 20281-20294.
55. Gurunathan, S., et al., Nanoparticle-mediated combination therapy: Two-in-one approach for cancer. International journal of molecular sciences, 2018. 19(10): p. 3264.
56. Avitabile, E., et al., How can nanotechnology help the fight against breast cancer? Nanoscale, 2018. 10(25): p. 11719-11731.
57. Vickers, N.J., Animal communication: when i’m calling you, will you answer too? Current biology, 2017. 27(14): p. R713-R715.
58. Dehghani Soufi, M., et al., Biolubricant production from edible and novel indigenous vegetable oils: mainstream methodology, and prospects and challenges in Iran. Biofuels, Bioproducts and Biorefining, 2019. 13(3): p. 838-849.
59. Allegra, A., et al., Nanoparticles in oncology: the new theragnostic molecules. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 2011. 11(7): p. 669-686.
60. Ashoori, Y., et al., Development and in vivo characterization of probiotic lysate-treated chitosan nanogel as a novel biocompatible formulation for wound healing. BioMed Research International, 2020. 2020: p. 1-9.
61. Mousavi, S.M., et al., Bioinorganic synthesis of polyrhodanine stabilized Fe3O4/Graphene oxide in microbial supernatant media for anticancer and antibacterial applications. Bioinorganic Chemistry and Applications, 2021. 2021.
62. Li, K.C., S. Guccione, and M.D. Bednarski, Combined vascular targeted imaging and therapy: a paradigm for personalized treatment. Journal of Cellular Biochemistry, 2002. 87(S39): p. 65-71.
63. Monfared, M., et al., Emerging frontiers in drug release control by core–shell nanofibers: A review. Drug metabolism reviews, 2019. 51(4): p. 589-611.
64. Parvin, N., et al., Removal of phenol and β-naphthol from aqueous solution by decorated graphene oxide with magnetic iron for modified polyrhodanine as nanocomposite adsorbents: Kinetic, equilibrium and thermodynamic studies. Reactive and functional polymers, 2020. 156: p. 104718.
65. Gaikwad, S., et al., Immobilized nanoparticles-mediated enzymatic hydrolysis of cellulose for clean sugar production: a novel approach. Current Nanoscience, 2019. 15(3): p. 296-303.
66. Abootalebi, S.N., et al., Antibacterial effects of green-synthesized silver nanoparticles using Ferula asafoetida against Acinetobacter baumannii isolated from the hospital environment and assessment of their cytotoxicity on the human cell lines. Journal of Nanomaterials, 2021. 2021: p. 1-12.
67. Tech, J.E.T., Investigating the activity of antioxidants activities content in Apiaceae and to study antimicrobial and insecticidal activity of antioxidant by using SPME Fiber assembly carboxen/polydimethylsiloxane (CAR/PDMS). Journal of Environmental Treatment Techniques, 2020. 8(1): p. 214-24.
68. Jain, K., Role of nanobiotechnology in developing personalized medicine for cancer. Technology in cancer research & treatment, 2005. 4(6): p. 645-650.
69. Mousavi, S.-M., et al., Bioactive agent-loaded electrospun nanofiber membranes for accelerating healing process: A review. Membranes, 2021. 11(9): p. 702.
70. Hashemi, S.A., et al., Ultra-precise label-free nanosensor based on integrated graphene with Au nanostars toward direct detection of IgG antibodies of SARS-CoV-2 in blood. Journal of Electroanalytical Chemistry, 2021. 894: p. 115341.
71. Jain, K.K., Nanotechnology in clinical laboratory diagnostics. Clinica chimica acta, 2005. 358(1-2): p. 37-54.
72. Jain, K.K., Nanobiotechnology in Molecular Diagnostics. Horizon Scientific Press, Norwich, UK, January, (in press), 2006.
73. Mousavi, S.M., et al., Recent biotechnological approaches for treatment of novel COVID-19: from bench to clinical trial. Drug Metabolism Reviews, 2021. 53(1): p. 141-170.
74. Ahmadi, S., et al., Anti-bacterial/fungal and anti-cancer performance of green synthesized Ag nanoparticles using summer savory extract. Journal of Experimental Nanoscience, 2020. 15(1): p. 363-380.
75. Yezhelyev, M., et al., O'. RM Nie Shuming. Lancet Onco, 2006. 7(8): p. 657-667.
76. Ahmadi, S., et al., Green synthesis of magnetic nanoparticles using Satureja hortensis essential oil toward superior antibacterial/fungal and anticancer performance. BioMed Research International, 2021. 2021.
77. Haq, A.I., et al., Impact of nanotechnology in breast cancer. Expert review of anticancer therapy, 2009. 9(8): p. 1021-1024.
78. Tanaka, T., et al., Nanotechnology for breast cancer therapy. Biomedical microdevices, 2009. 11(1): p. 49-63.
79. Yezhelyev, M.V., et al., Emerging use of nanoparticles in diagnosis and treatment of breast cancer. The lancet oncology, 2006. 7(8): p. 657-667.
80. Ferrari, M., Cancer nanotechnology: opportunities and challenges. Nature reviews cancer, 2005. 5(3): p. 161-171.
81. Wang, X., et al., Application of nanotechnology in cancer therapy and imaging. CA: a cancer journal for clinicians, 2008. 58(2): p. 97-110.
82. Jain, K., Nanobiotechnology: technologies, markets and companies. 2010, Basel, Switzerland: Jain PharmaBiotech Publications.
83. Therapy, T., National Breast Cancer Foundation. Inc, 2019.
84. Mousavi, S.M., et al., Development of graphene based nanocomposites towards medical and biological applications. Artificial cells, nanomedicine, and biotechnology, 2020. 48(1): p. 1189-1205.
85. Panariti, A., G. Miserocchi, and I. Rivolta, The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions? Nanotechnology, science and applications, 2012. 5: p. 87.
86. Cho, E.C., Y. Liu, and Y. Xia, A simple spectroscopic method for differentiating cellular uptakes of gold nanospheres and nanorods from their mixtures. Angewandte Chemie, 2010. 122(11): p. 2020-2024.
87. Ross, C., et al., Liposome delivery systems for the treatment of Alzheimer’s disease. International journal of nanomedicine, 2018. 13: p. 8507.
88. Ye, B.-l., et al., Chitosan-coated doxorubicin nano-particles drug delivery system inhibits cell growth of liver cancer via p53/PRC1 pathway. Biochemical and biophysical research communications, 2018. 495(1): p. 414-420.
89. Jain, A.K. and S. Jain, Advances in oral delivery of anti-cancer prodrugs. Expert opinion on drug delivery, 2016. 13(12): p. 1759-1775.
90. Mei, L., et al., Pharmaceutical nanotechnology for oral delivery of anticancer drugs. Advanced drug delivery reviews, 2013. 65(6): p. 880-890.
91. Thanki, K., et al., Oral delivery of anticancer drugs: challenges and opportunities. Journal of controlled release, 2013. 170(1): p. 15-40.
92. Mohammed, M.A., et al., An overview of chitosan nanoparticles and its application in non-parenteral drug delivery. Pharmaceutics, 2017. 9(4): p. 53.
93. Kang, X., et al., Magnesium lithospermate B loaded PEGylated solid lipid nanoparticles for improved oral bioavailability. Colloids and Surfaces B: Biointerfaces, 2018. 161: p. 597-605.
94. Ibrahim, S., et al., Curcumin marinosomes as promising nano-drug delivery system for lung cancer. International journal of pharmaceutics, 2018. 540(1-2): p. 40-49.
95. Fathi Karkan, S., et al., Magnetic nanoparticles in cancer diagnosis and treatment: a review. Artificial cells, nanomedicine, and biotechnology, 2017. 45(1): p. 1-5.
96. England, C.G., A.M. Gobin, and H.B. Frieboes, Evaluation of uptake and distribution of gold nanoparticles in solid tumors. The European Physical Journal Plus, 2015. 130(11): p. 1-16.
97. Ali, H., et al., Red fluorescent carbon nanoparticle-based cell imaging probe. ACS Applied Materials & Interfaces, 2016. 8(14): p. 9305-9313.
98. Arunrut, N., et al., Sensitive visual detection of AHPND bacteria using loop-mediated isothermal amplification combined with DNA-functionalized gold nanoparticles as probes. PLoS One, 2016. 11(3): p. e0151769.
99. Nadimi, A.E., et al., Nano-scale drug delivery systems for antiarrhythmic agents. European journal of medicinal chemistry, 2018. 157: p. 1153-1163.
100. Trédan, O., et al., Drug resistance and the solid tumor microenvironment. Journal of the National Cancer Institute, 2007. 99(19): p. 1441-1454.
101. Minchinton, A.I. and I.F. Tannock, Drug penetration in solid tumours. Nature Reviews Cancer, 2006. 6(8): p. 583-592.
102. Primeau, A.J., et al., The distribution of the anticancer drug Doxorubicin in relation to blood vessels in solid tumors. Clinical Cancer Research, 2005. 11(24): p. 8782-8788.
103. Bosslet, K., et al., Elucidation of the mechanism enabling tumor selective prodrug monotherapy. Cancer research, 1998. 58(6): p. 1195-1201.
104. Lu, R.-M., et al., Targeted drug delivery systems mediated by a novel Peptide in breast cancer therapy and imaging. PloS one, 2013. 8(6): p. e66128.
105. Maeda, H., H. Nakamura, and J. Fang, The EPR effect for macromolecular drug delivery to solid tumors: Improvement of tumor uptake, lowering of systemic toxicity, and distinct tumor imaging in vivo. Advanced drug delivery reviews, 2013. 65(1): p. 71-79.
106. Al-Abd, A.M., et al., Pharmacokinetic strategies to improve drug penetration and entrapment within solid tumors. Journal of Controlled Release, 2015. 219: p. 269-277.
107. Khawar, I.A., J.H. Kim, and H.-J. Kuh, Improving drug delivery to solid tumors: priming the tumor microenvironment. Journal of Controlled Release, 2015. 201: p. 78-89.
108. Rebucci, M. and C. Michiels, Molecular aspects of cancer cell resistance to chemotherapy. Biochemical pharmacology, 2013. 85(9): p. 1219-1226.
109. Mimeault, M., et al., Functions of normal and malignant prostatic stem/progenitor cells in tissue regeneration and cancer progression and novel targeting therapies. Endocrine reviews, 2008. 29(2): p. 234-252.
110. Shah, V., et al., Targeted Nanomedicine for Suppression of CD44 and Simultaneous Cell Death Induction in Ovarian Cancer: An Optimal Delivery of siRNA and Anticancer DrugTargeted Suppression of CD44 Protein and Cell Death Induction. Clinical Cancer Research, 2013. 19(22): p. 6193-6204.
111. Dreher, M.R., et al., Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers. Journal of the National Cancer Institute, 2006. 98(5): p. 335-344.
How to Cite
Madadi Atmian Sofla F, Babapoor A. Recent advances in nano-biotechnology for breast cancer therapy. AANBT [Internet]. 20Dec.2022 [cited 30Sep.2023];3(4):1-. Available from: