Bioenzyme based on nanobiotechnology for cancer treatment

A mini-review

  • Seyyed Navid Mousavinejad Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Keywords: Bioenzyme, NanoTechnology, Cancer Therapy



     Biological enzymes, also known as organic catalysts, play an effective role in the treatment of cancer, especially when combined with nanoparticles using nanobiotechnology methods, their anti-tumor effect improves. Nanoparticle-based bioenzymes play an important role in improving cancer treatment by affecting tumor tissue microenvironment such as pH, glucose concentration, hypoxia and redox reaction. Moreover, by degrading the extracellular matrix of tumor tissue, nanoparticle-based enzymes cause increased accumulation of immune cells in this area, thus enhancing the efficacy of treatments based on chemotherapy, photothermal therapy, radiotherapy, and immunotherapy.This mini-review summarizes the recent progress in the field of enhancing cancer treatment methods based on nanoparticle-conjugated enzymes, presents the structure of nanoparticle-based bioenzymes and the various applications of bioenzymes, advantages and disadvantages, challenges, and conclusions.

Keywords: Bioenzyme, NanoTechnology, Cancer Therapy


Download data is not yet available.


1. Moss, M.L., et al., ADAM10 as a target for anti-cancer therapy. Current pharmaceutical biotechnology, 2008. 9(1): p. 2-8.
2. Xie, Z., et al., Emerging combination strategies with phototherapy in cancer nanomedicine. Chem Soc Rev, 2020. 49(22): p. 8065-8087.
3. Shang, T., et al., Nanomedicine-based tumor photothermal therapy synergized immunotherapy. Biomater Sci, 2020. 8(19): p. 5241-5259.
4. Jin, S., et al., Nanoscale dual-enzyme cascade metal-organic frameworks through biomimetic mineralization as ROS generators for synergistic cancer therapy. J Mater Chem B, 2020. 8(21): p. 4620-4626.
5. Mousavi, S.M., et al., Bioactive Graphene Quantum Dots Based Polymer Composite for Biomedical Applications. Polymers, 2022. 14(3): p. 617.
6. Mousavi, S.M., et al., Plasma-Enabled Smart Nanoexosome Platform as Emerging Immunopathogenesis for Clinical Viral Infection. Pharmaceutics, 2022. 14(5): p. 1054.
7. Thompson, C.B., et al., Enzymatic depletion of tumor hyaluronan induces antitumor responses in preclinical animal models. Mol Cancer Ther, 2010. 9(11): p. 3052-64.
8. Jacobetz, M.A., et al., Hyaluronan impairs vascular function and drug delivery in a mouse model of pancreatic cancer. Gut, 2013. 62(1): p. 112-20.
9. Li, X., et al., Parallel Accumulation of Tumor Hyaluronan, Collagen, and Other Drivers of Tumor Progression. Clin Cancer Res, 2018. 24(19): p. 4798-4807.
10. Alipour, A. and M.Y. Kalashgarani, Nano Protein and Peptides for Drug Delivery and Anticancer Agents. Advances in Applied NanoBio-Technologies, 2022. 3(1): p. 60-64.
11. Mousavi, S.M., et al., Recent Advances in Plasma-Engineered Polymers for Biomarker-Based Viral Detection and Highly Multiplexed Analysis. Biosensors, 2022. 12(5): p. 286.
12. Bai, J., Y. Liu, and X. Jiang, Multifunctional PEG-GO/CuS nanocomposites for near-infrared chemo-photothermal therapy. Biomaterials, 2014. 35(22): p. 5805-13.
13. Lucky, S.S., K.C. Soo, and Y. Zhang, Nanoparticles in photodynamic therapy. Chem Rev, 2015. 115(4): p. 1990-2042.
14. Mei, X., et al., Highly dispersed nano-enzyme triggered intracellular catalytic reaction toward cancer specific therapy. Biomaterials, 2020. 258: p. 120257.
15. Ding, Y., et al., Tumor microenvironment responsive polypeptide-based supramolecular nanoprodrugs for combination therapy. Acta Biomater, 2022. 146: p. 396-405.
16. Meng, X., et al., Fenton reaction-based nanomedicine in cancer chemodynamic and synergistic therapy. Applied Materials Today, 2020. 21: p. 100864.
17. Kalashgarani, M.Y. and A. Babapoor, Application of nano-antibiotics in the diagnosis and treatment of infectious diseases. Advances in Applied NanoBio-Technologies, 2022. 3(1): p. 22-35.
18. Mousavi, S.M., et al., Highly sensitive flexible SERS-based sensing platform for detection of COVID-19. Biosensors, 2022. 12(7): p. 466.
19. Taipa, M., P. Fernandes, and C. de Carvalho, Production and Purification of Therapeutic Enzymes. Adv Exp Med Biol, 2019. 1148: p. 1-24.
20. Liu, C., et al., A boronic acid-rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing. Sci Adv, 2019. 5(6): p. eaaw8922.
21. Rui, Y., et al., Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing. Science Advances, 2019. 5(12): p. eaay3255.
22. Kazemi, K., Y. Ghahramani, and M.Y. Kalashgrani, Nano biofilms: An emerging biotechnology applications. Advances in Applied NanoBio-Technologies, 2022: p. 8-15.
23. Kalashgrani, M.Y. and N. Javanmardi, Multifunctional Gold nanoparticle: As novel agents for cancer treatment. Advances in Applied NanoBio-Technologies, 2022: p. 43-48.
24. Mousavi, S.M., et al., Recent Advances in Inflammatory Diagnosis with Graphene Quantum Dots Enhanced SERS Detection. Biosensors, 2022. 12(7): p. 461.
25. Norouzi, M., et al., Clinical applications of nanomedicine in cancer therapy. Drug Discov Today, 2020. 25(1): p. 107-125.
26. Pérez-Herrero, E. and A. Fernández-Medarde, Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy. Eur J Pharm Biopharm, 2015. 93: p. 52-79.
27. Wicki, A., et al., Nanomedicine in cancer therapy: challenges, opportunities, and clinical applications. J Control Release, 2015. 200: p. 138-57.
28. Blanco, E., et al., Nanomedicine in cancer therapy: innovative trends and prospects. Cancer Sci, 2011. 102(7): p. 1247-52.
29. Mozafari, M.R., et al., Role of nanocarrier systems in cancer nanotherapy. J Liposome Res, 2009. 19(4): p. 310-21.
30. Pauwels, E.K. and P. Erba, Towards the use of nanoparticles in cancer therapy and imaging. Drug News Perspect, 2007. 20(4): p. 213-20.
31. Kalashgrani, M.Y., F.F. Nejad, and V. Rahmanian, Carbon Quantum Dots Platforms: As nano therapeutic for Biomedical Applications. Advances in Applied NanoBio-Technologies, 2022: p. 38-42.
32. Mousavi, S.M., et al., Biomedical Applications of an Ultra-Sensitive Surface Plasmon Resonance Biosensor Based on Smart MXene Quantum Dots (SMQDs). Biosensors, 2022. 12(9): p. 743.
33. Mitchell, M.J., et al., Engineering precision nanoparticles for drug delivery. Nat Rev Drug Discov, 2021. 20(2): p. 101-124.
34. Kalaydina, R.V., et al., Recent advances in "smart" delivery systems for extended drug release in cancer therapy. Int J Nanomedicine, 2018. 13: p. 4727-4745.
35. Liu, M.C., et al., Folate receptor-targeted liposomes loaded with a diacid metabolite of norcantharidin enhance antitumor potency for H22 hepatocellular carcinoma both in vitro and in vivo. Int J Nanomedicine, 2016. 11: p. 1395-412.
36. Wang, R., et al., Preclinical Study using Malat1 Small Interfering RNA or Androgen Receptor Splicing Variant 7 Degradation Enhancer ASC-J9(®) to Suppress Enzalutamide-resistant Prostate Cancer Progression. Eur Urol, 2017. 72(5): p. 835-844.
37. Cheng, R., et al., Dual and multi-stimuli responsive polymeric nanoparticles for programmed site-specific drug delivery. Biomaterials, 2013. 34(14): p. 3647-57.
38. Mousavi, S.M., et al., The Pivotal Role of Quantum Dots-Based Biomarkers Integrated with Ultra-Sensitive Probes for Multiplex Detection of Human Viral Infections. Pharmaceuticals, 2022. 15(7): p. 880.
39. Wang, H., et al., Homotypic targeting upconversion nano-reactor for cascade cancer starvation and deep-tissue phototherapy. Biomaterials, 2020. 235: p. 119765.
40. Kalashgrani, M.Y., et al., Recent Advances in Multifunctional magnetic nano platform for Biomedical Applications: A mini review. Advances in Applied NanoBio-Technologies, 2022: p. 31-37.
41. Mousavi, S., et al., Biodegradation study of nanocomposites of phenol novolac epoxy/unsaturated polyester resin/egg shell nanoparticles using natural polymers. Journal of Materials, 2015. 2015: p. 1-6.
42. Liao, R., et al., Enzymatic protection and biocompatibility screening of enzyme-loaded polymeric nanoparticles for neurotherapeutic applications. Biomaterials, 2020. 257: p. 120238.
43. Ahmed, O.A.A. and S.M. Badr-Eldin, Biodegradable self-assembled nanoparticles of PEG-PLGA amphiphilic diblock copolymer as a promising stealth system for augmented vinpocetine brain delivery. Int J Pharm, 2020. 588: p. 119778.
44. Ke, W., et al., Therapeutic Polymersome Nanoreactors with Tumor-Specific Activable Cascade Reactions for Cooperative Cancer Therapy. ACS Nano, 2019. 13(2): p. 2357-2369.
45. Hashemi, S.A., et al., Superior X-ray radiation shielding effectiveness of biocompatible polyaniline reinforced with hybrid graphene oxide-iron tungsten nitride flakes. Polymers, 2020. 12(6): p. 1407.
46. Mousavi, S., et al., Modification of the epoxy resin mechanical and thermal properties with silicon acrylate and montmorillonite nanoparticles. Polymers from Renewable Resources, 2016. 7(3): p. 101-113.
47. Liang, R., et al., Triggering Sequential Catalytic Fenton Reaction on 2D MXenes for Hyperthermia-Augmented Synergistic Nanocatalytic Cancer Therapy. ACS Appl Mater Interfaces, 2019. 11(46): p. 42917-42931.
48. Du, K., et al., Encapsulation of glucose oxidase in Fe(III)/tannic acid nanocomposites for effective tumor ablation via Fenton reaction. Nanotechnology, 2020. 31(1): p. 015101.
49. Meng, X., et al., A metal-phenolic network-based multifunctional nanocomposite with pH-responsive ROS generation and drug release for synergistic chemodynamic/photothermal/chemo-therapy. J Mater Chem B, 2020. 8(10): p. 2177-2188.
50. Ranji-Burachaloo, H., et al., Combined Fenton and starvation therapies using hemoglobin and glucose oxidase. Nanoscale, 2019. 11(12): p. 5705-5716.
51. Hashemi, S.A., et al., Reinforced polypyrrole with 2D graphene flakes decorated with interconnected nickel-tungsten metal oxide complex toward superiorly stable supercapacitor. Chemical Engineering Journal, 2021. 418: p. 129396.
52. Mousavi, S., et al., Improved morphology and properties of nanocomposites, linear low density polyethylene, ethylene-co-vinyl acetate and nano clay particles by electron beam. Polymers from Renewable Resources, 2016. 7(4): p. 135-153.
53. Si, X., et al., Hypoxia-sensitive supramolecular nanogels for the cytosolic delivery of ribonuclease A as a breast cancer therapeutic. J Control Release, 2020. 320: p. 83-95.
54. Zhou, H., et al., A polypeptide based podophyllotoxin conjugate for the treatment of multi drug resistant breast cancer with enhanced efficiency and minimal toxicity. Acta Biomater, 2018. 73: p. 388-399.
55. Choi, J.H., et al., Intracellular delivery and anti-cancer effect of self-assembled heparin-Pluronic nanogels with RNase A. J Control Release, 2010. 147(3): p. 420-7.
56. Si, X., et al., Glucose and pH Dual-Responsive Nanogels for Efficient Protein Delivery. Macromol Biosci, 2019. 19(9): p. e1900148.
57. Mohammadi, M., L. Arabi, and M. Alibolandi, Doxorubicin-loaded composite nanogels for cancer treatment. J Control Release, 2020. 328: p. 171-191.
58. Mousavi, S.M., et al., Development of graphene based nanocomposites towards medical and biological applications. Artificial cells, nanomedicine, and biotechnology, 2020. 48(1): p. 1189-1205.
59. Hashemi, S.A., et al., Electrified single‐walled carbon nanotube/epoxy nanocomposite via vacuum shock technique: Effect of alignment on electrical conductivity and electromagnetic interference shielding. Polymer Composites, 2018. 39(S2): p. E1139-E1148.
60. Li, M., et al., Tumor extracellular matrix modulating strategies for enhanced antitumor therapy of nanomedicines. Mater Today Bio, 2022. 16: p. 100364.
61. Xu, X., et al., Nanomedicine Strategies to Circumvent Intratumor Extracellular Matrix Barriers for Cancer Therapy. Adv Healthc Mater, 2022. 11(1): p. e2101428.
62. Cabral, H., H. Kinoh, and K. Kataoka, Tumor-Targeted Nanomedicine for Immunotherapy. Acc Chem Res, 2020. 53(12): p. 2765-2776.
63. Durymanov, M.O., A.A. Rosenkranz, and A.S. Sobolev, Current Approaches for Improving Intratumoral Accumulation and Distribution of Nanomedicines. Theranostics, 2015. 5(9): p. 1007-20.
64. Mousavi, S.M., et al., Asymmetric membranes: a potential scaffold for wound healing applications. Symmetry, 2020. 12(7): p. 1100.
65. Sun, Q., et al., Enhancing Tumor Penetration of Nanomedicines. Biomacromolecules, 2017. 18(5): p. 1449-1459.
66. Mousavi, S.M., et al., Development of clay nanoparticles toward bio and medical applications. 2018: IntechOpen London, UK.
67. Zhu, J., et al., Surface-Charge-Switchable Nanoclusters for Magnetic Resonance Imaging-Guided and Glutathione Depletion-Enhanced Photodynamic Therapy. ACS Nano, 2020. 14(9): p. 11225-11237.
68. Dolmans, D.E., D. Fukumura, and R.K. Jain, Photodynamic therapy for cancer. Nature reviews cancer, 2003. 3(5): p. 380-387.
69. Agostinis, P., et al., Photodynamic therapy of cancer: an update. CA: a cancer journal for clinicians, 2011. 61(4): p. 250-281.
70. Lismont, M., L. Dreesen, and S. Wuttke, Metal‐organic framework nanoparticles in photodynamic therapy: current status and perspectives. Advanced Functional Materials, 2017. 27(14): p. 1606314.
71. Wang, Y., et al., A step‐by‐step multiple stimuli‐responsive nanoplatform for enhancing combined chemo‐photodynamic therapy. Advanced Materials, 2017. 29(12): p. 1605357.
72. Azhdari, R., et al., Decorated graphene with aluminum fumarate metal organic framework as a superior non-toxic agent for efficient removal of Congo Red dye from wastewater. Journal of Environmental Chemical Engineering, 2019. 7(6): p. 103437.
73. Mousavi, S.M., et al., Green synthesis of supermagnetic Fe3O4–MgO nanoparticles via Nutmeg essential oil toward superior anti-bacterial and anti-fungal performance. Journal of Drug Delivery Science and Technology, 2019. 54: p. 101352.
74. Wu, F., et al., Enhanced Cancer Starvation Therapy Based on Glucose Oxidase/3-Methyladenine-Loaded Dendritic Mesoporous OrganoSilicon Nanoparticles. Biomolecules, 2021. 11(9).
75. Marx, J., A boost for tumor starvation. 2003, American Association for the Advancement of Science.
76. Li, S., et al., A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo. Nature biotechnology, 2018. 36(3): p. 258-264.
77. Krock, B.L., N. Skuli, and M.C. Simon, Hypoxia-induced angiogenesis: good and evil. Genes & cancer, 2011. 2(12): p. 1117-1133.
78. Pedraza, E., et al., Preventing hypoxia-induced cell death in beta cells and islets via hydrolytically activated, oxygen-generating biomaterials. Proceedings of the National Academy of Sciences, 2012. 109(11): p. 4245-4250.
79. Koppenol, W.H., P.L. Bounds, and C.V. Dang, Otto Warburg's contributions to current concepts of cancer metabolism. Nature Reviews Cancer, 2011. 11(5): p. 325-337.
80. Mousavi, S.M., et al., Recent progress in chemical composition, production, and pharmaceutical effects of kombucha beverage: a complementary and alternative medicine. Evidence-Based Complementary and Alternative Medicine, 2020. 2020.
81. Hashemi, S.A. and S.M. Mousavi, Effect of bubble based degradation on the physical properties of Single Wall Carbon Nanotube/Epoxy Resin composite and new approach in bubbles reduction. Composites Part A: Applied Science and Manufacturing, 2016. 90: p. 457-469.
82. Lu, R., et al., Skillfully collaborating chemosynthesis with GOx-enabled tumor survival microenvironment deteriorating strategy for amplified chemotherapy and enhanced tumor ablation. Biomaterials Science, 2021. 9.
83. Li, Z., et al., Glucose Metabolism Intervention-Facilitated Nanomedicine Therapy. International Journal of Nanomedicine, 2022. Volume 17: p. 2707-2731.
84. Feng, L., et al., Magnetic Targeting, Tumor Microenvironment-Responsive Intelligent Nanocatalysts for Enhanced Tumor Ablation. ACS Nano, 2018. 12(11): p. 11000-11012.
85. Bonet-Aleta, J., J. Calzada-Funes, and J.L. Hueso, Manganese oxide nano-platforms in cancer therapy: Recent advances on the development of synergistic strategies targeting the tumor microenvironment. Applied Materials Today, 2022. 29: p. 101628.
86. Chen, X., et al., Metal-phenolic networks-encapsulated cascade amplification delivery nanoparticles overcoming cancer drug resistance via combined starvation/chemodynamic/chemo therapy. Chemical Engineering Journal, 2022. 442: p. 136221.
87. Mousavi, S.M., et al., Data on cytotoxic and antibacterial activity of synthesized Fe3O4 nanoparticles using Malva sylvestris. Data in brief, 2020. 28: p. 104929.
How to Cite
Mousavinejad SN. Bioenzyme based on nanobiotechnology for cancer treatment. AANBT [Internet]. 20Sep.2022 [cited 29Sep.2022];3(3):7-1. Available from: